Lab of Molecular Imaging and Stem Cell Therapy
Nankai University School of Medicine
Tianjin 300071
China
Eperiment apparatus
Sorting flow cytometer
(1) Laser configuration: 488 nm, 640 nm, 355 nm, 405 nm (2) Monoclonal sorting components (3) 4-way sorting, the speed can reach 100,000 cells/second (4) Sorting purity> 98%; Cell viability > 90% (5) Detection of fluorescence: FITC, PE, PI, PerCP, PerCP-CY5.5, APC, AF700, APC-CY7, Hoechst blue, Hoechst red, INDO1, BV421, BV510, BV605, BV650, BV785
FE_SEM
Resolution: 0.8nm (15kV)1.2nm (1kV) Magnification: 25 to 1,000,000x Accelerating Voltage: 0.01 to 30kV Beam Intensity: 200 nA (15kV) Electron Gun: Immersion Thermal Field Emission Electron Gun Objective Design: Super Hybrid Objective Sample stage: 5-axis motor drive
LSM 880 with Airyscan
Laser: 405nm, 458nm, 488nm, 514nm, 543nm, 633nm; Super resolution: lateral resolution ≤140nm, axial resolution ≤400nm; microscope: eyepiece 10X, objective 10X, 20X, 40X, 63X (oil lens); Live cell culture: carbon dioxide concentration, temperature and humidity can be controlled.
IVIS Lumina II
Ultra-high sensitivity CCD, the imaging field of view is 5 to 12.5 cm; the band covers 515-875 nm, in addition to bioluminescence, it can detect GFP, RFP, DsRed, Cy5.5, ICG common fluorescent dyes or fluorescent probes and quantum dot markers;
Talos F200C
Information resolution ≤0.15nm 20-200kV accelerating voltage C-TWIN pole piece Piezoelectric ceramic drive five-axis sample stage 16 million pixel CMOS camera Falcon direct electron detector Completely oil-free vacuum system Freezing transfer sample holder Automatic cold box
Olympus IX81
1. Objective lens: equipped with 10 times, L20 times, L40 times long working distance, L60 times long working distance, L100 times long working distance 2. Equipped with EMCCD and live cell culture system 3. Equipped with multi-color filter blocks, which can realize multi-channel imaging
Nankai University School of Medicine
Tianjin 300071
China